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10.1038/nature12040 189 SothM

Mitigation strategies include strict sun protection during treatment, using moderate rather than aggressive microneedling depths, incorporating niacinamide which helps prevent PIH, considering patch testing before full-face treatment, and being prepared to pause treatment if significant PIH develops

The oxidative stress model was validated by staining RBCs with the DCF-DA dye (35845, Sigma-Aldrich), which was sensitive to the presence of ROS by flow cytometry analysis (Supplementary Fig