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Three thiamine dependant enzymes PDE3, PDE1, AKGDHE1 and Transketolase were determined to be dysfunctional at baseline in type 2 microalbuminuric diabetic patients in comparison to normal healthy controls, and improved in both activity and gene expression with high dose thiamine therapy While importantly no hepatic or renal adverse effects were encountered prior, during therapy or as a residual effect, post washout thus fortifying the previously established human safety track record of thiamine

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