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Construct highly reproducible calibration curves from serial dilutions, ensuring accuracy in the lower quantitation range

For gut conditions, oral may be preferable to injection

Chronic unpredictable stress, considered a model of depression, results in a reduction in dendritic lengths and branching of apical dendrites, the loss of functional synaptic spines, and the reduction of dendritic complexity in PFC neurons and in the hippocampal CA3 pyramidal neuronal cell layer (McEwen et al., 2012

Standard dosing protocol Goal: Maximize GH pulses while keeping side effects manageable Dose: 100 mcg per injection, 2-3 times daily Timing: Dose 1: Immediately upon waking (fasted) Dose 2: Immediately post-workout Dose 3: 30 minutes before bed Administration route: Subcutaneous injection (preferred) or intramuscular