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Starting low and increasing gradually gives your body time to adjust and helps reduce the chance of nausea and other side effects

Briefly, after the mice were anaesthetized with 60 mg/kg sodium pentobarbital, a stereotactic device was used to implant electrodes into the cerebral cortex on both sides of the bregma

The gradient program was run as follows: 0100% methanol, 030 min, and 100% methanol, 10 min