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FIGURE 4 The BHBA-Induced Changes of H2AK119ub and H3K4me3 Depend on Different Ssignals To further determine the roles of L-type VSCCs and Ca 2+ /CaM in regulating the BHBA-mediated increase of H3K4me3, hippocampal neurons were pre-treated with either manidipine or KN-62

Briefly, after blocking with 10% goat serum, 20 m cryosections were incubated with goat anti-mouse IgG conjugated with Alexa488 (1:50, Life Technologies) for 20 h at 4 C