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Finally, the 3-Cy3 labelling reaction was performed overnight at 4C in 20 l containing 100 pmol unlabeled RNA, 0.5 mM ATP, 200 pmol pCp-Cy3 (Jena Bioscience), 10 U RiboLock RNase Inhibitor (Thermo Fisher Scientific), 1x Ligation Buffer and 10 U T4 RNA Ligase (Thermo Fisher Scientific)

Muscle damage , which is characterised by increased protein turnover in both muscles and the rest of the body, and the oxidation of amino acids during and after exercise increase a persons need for protein

Control group: FLS were not treated except for medium replacement