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paracasei LMG-S-26420 viable counts were performed following the guidelines provided in the document ISTISAN Reports 2008/36, traditional microbiological methods and molecular methods for the analysis of food supplements based on or with probiotics for human use. For the Lactobacillus genus, this document specifies the use of MRS agar (BD, Difco, Italy) plates inoculated by spreading and incubating for 72 h at 37 C under anaerobic conditions

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American Thoracic Society

These include vitamin B 12 deficiency, mutations of the MTHFR gene, and epigenetic modifications that alter expression of other key enzymes involved in folate metabolism