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Volumetric changes from baseline to follow-up in MRI scans were evaluated using both regional volume analysis and voxel-based morphometric analysis

Splitting daily doses into two administrations (morning and evening) maintains more consistent circulating levels

The effect of experimental compounds on voluntary wheel running was monitored by randomizing mice to receive once-daily s.c

Four experimental groupsinsulin administered via subcutaneous injection (ins-sc), insulin solution, insulin-loaded nanoparticles containing 2 w/v% chitosan, and insulin-unloaded nanoparticleswere compared to determine their antihyperglycemic effects in the diabetic albino Wistar mouse model