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Plasma levels of 8-isoprostane (# 516351, Cayman Chemical, USA), and hs-CRP (# EKF57936, Biomatik, Canada) were determined using commercially available ELISA kits, according to the manufacturers instructions

doi: 10.3389/fmolb.2025.1622186 Received 02 May 2025 Accepted 02 June 2025 Published 26 June 2025 Volume 12 - 2025 Edited by Roberta Marongiu, Cornell University, United States Reviewed by Larance Ronsard, Ragon Institute, United States Jorge Felipe Argenta Model, Federal University of Rio Grande do Sul, Brazil Updates Copyright 2025 Lizcano, Sanabria and Aviles

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Taking NAD+ and glutathione together creates a reinforcing cycle of cellular protection because NAD+ metabolism generates NADPH for glutathione recycling while glutathione protects against oxidative damage that depletes NAD+