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Long-range Coulomb interactions were handled using the particle mesh Ewald summation method (PME) 54 by setting the mesh spacing to 1.0

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The FDA scientists concluded that "the peptides being considered lacked the necessary evidence to conclude that they were safe and effective for treating the conditions they were purported to help treat," according to The Hill

CzEcp2, CzNIS1a and CzNIS1b were subsequently transiently expressed in model plant systems and shown to trigger non-host resistance, suggesting they target conserved structures or processes to facilitate pathogen colonization and growth during the switch to necrotrophy