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Cell Death Dis 5(6):e1299

Oxidative stress, a term refers to the injurious results in living organisms due to an imbalance favoring oxidants over antioxidants ( in vivo interactions between oxygen and organic molecules

The removal of the staining working solution and subsequent rinsing of the cells with PBS was imperative, pre-warmed PBS was added and the cells were incubated for 5 min and photographed and documented under an inverted fluorescence microscope