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After incubation with horseradish-peroxidase-conjugated secondary antibodies, signals were detected by Western Lightning Plus enhanced chemiluminescence Substrate (PerkinElmer, NEL103E001EA)

Overexpression of CHAC1 in prostate cancer cell lines DU145 and 22RV1 significantly reduces cell viability and GSH levels, while its knockdown increases viability in DU145 cells

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In addition, a recent study also revealed that the liver-specific Ulk1 and Ulk2 double knockout mice, where Ulk1 and Ulk2 were key components of the unc-51-like autophagy activating kinase 1 (ULK1) complex that functions upstream of the autophagy pathway, were more resistant to APAP-induced liver injury