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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)

Most patients notice diarrhea peaking three to seven days after a dose increase, with improvement over one to two weeks as the intestinal system adapts

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For any transdermal medication patch, the appropriate action if detachment occurs would be detailed in the specific product's Patient Information Leaflet (PIL)