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Supraphysiological testosterone concentrations have been shown to reduce endothelial nitric oxide synthase expression and nitric oxide bioavailability, thereby favouring vasoconstriction and increased arterial stiffness (73)

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After incubated at 20 C overnight, the samples were centrifugated and the amount of TP-10 was quantified by HPLC-MS/MS according to the chromatographic conditions described above

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