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Immunohistochemistry (IHC) Detection of insulin (anti-Insulin Novus NBP1-19803 antibody, RRID: AB_1642425, dilution 1:200) and GRK2 (anti-GRK2 PF2 antibody [92], dilution 1:500) was performed in mouse pancreatic tissue sections deparaffinized and rehydrated prior to antigen retrieval using citrate buffer (10 mM Sodium Citrate, 0.05% Tween 20, pH 6.0), microwave-heated to boiling temperature twice

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