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In general, yeasts are grown in the laboratory on solid growth media or in liquid broths

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Materials and Methods Cell Culture HeLa cells were cultured in DMEM +FBS [DMEM without pyruvate and glucose (ThermoFisher, 11966025) supplemented with 25 mM glucose, 10% non-dialyzed FBS (Sigma, F2442) and 1% penicillin/streptomycin] at 37C in 5% CO 2