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& Gong, T

Study on the quaternary structure suggested that rhGPx3 mutant existed as a monomer in solution, which is different from native tetrameric GPx3

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Mass range of m / z 300 to 1650 (Q-Exactive Plus) or 375 to 1500 (Q-Exactive HF) was acquired with a resolution of 70000 (Q-Exactive Plus) or 120,000 (Q-Exactive HF) for full scan, followed by up to ten (Q-Exactive Plus) or fifteen (Q-Exactive HF) high energy collision dissociation (HCD) MS/MS scans of the most intense at least doubly charged ions using a resolution of 17500 (Q-Exactive Plus) or 15000 (Q-Exactive HF) and a NCE energy of 25% (Q-Exactive Plus) or 27% (QExactive HF)