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Immunoblotting Protein extracts from adipose tissue mitochondria or cells were prepared using homogenization in radioimmunoprecipitation assay buffer containing protease inhibitors (Sigma-Aldrich)

144 Based on estimates, under physiological conditions, one-third of the HSA exists as disulfides mixed with cysteine, homocysteine, or glutathione (HSA-S-S-R), whereas the rest of the HSA is in a reduced form with a free thiol in the Cys-34 residue (HSA-SH)

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Strict vegans who take no animal or dairy produce may not eat enough vitamin B12