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LEfSe is known to reduce false positives and can highlight biological consistency across subclasses, while the KruskalWallis test used alone can achieve a lower false-negative rate compared to LEfSe 38

doi: 10.3389/fimmu.2016.00035 118 LiPJiangMLiKLiHZhouYXiaoXet al

Behavioral severity was measured using the Childhood Autism Rating Scale (CARS), and adherence to treatment was tracked using parent-reported measures