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A significant negative latitude-dependent relationship was detected for carriage of the T allele, with occurrence of the cSHMT-C1420T polymorphic allele decreasing with increase in latitude ( r 2 =0.73, p 3R frequency in reported African and Eurasia studies found a significant negative relationship between latitude and frequency of the 3R allele ( r 2 =0.12, p =0.039) (Fig

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Inactivate the enzyme by adding 0.3 mM PMSF (final concentration) to the sample