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Methods All reagents and solvents were purchased from commercial sources and used without further purification

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Although high ROS levels cause cellular damage and dysfunction, a low basal level of ROS is considered advantageous for maintaining cellular proliferation, differentiation, and survival [31, 32]

In mice model single dosage of 150 mg per kg of cyclophosphamide was administered intraperitoneally then different icariin doses were administered intraperitoneally such as 5 mg, 25 mg, and 50 mg per kg were shown to reduce visceral reflexes and nociception