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RNA extraction and real-time PCR Following instructions provided by the manufacturer, total RNA was extracted from cells and mouse adipose tissue using an RNA extraction kit (Omega, USA)

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In the context of lipid metabolism, targeting pathways such as ether lipid synthesis, fatty acid synthase (FASN), or the ACLY/ACC axis has been shown to induce mitochondrial stress and ROS accumulation, thereby enhancing apoptotic responses [144]

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