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Following the 10 min incubation period, we centrifuged the 5% SSA plasma mixture for 10 min at 14,000 rpm in a refrigerated centrifuge at 4 o C

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Also, during STEP 5, a study to assess efficacy of semaglutide with a follow-up of 2 years, there is a post-hoc analysis that demonstrates a better performance in the self-reported Control of Eating Questionnaire(CoEQ) to measure changes in appetite versus placebo, with lower cravings for dairy and starchy foods and less desire for salty and spicy food at short-term (20 weeks) but also as a maintenance therapy at weeks 52 and 104 (178)