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In further studies, only the formation of [14C]carbon dioxide from [14C-1]glucose was measured, with the addition of: sodium ascorbate (which undergoes a non-enzymic reaction in air to produce hydrogen peroxide) acetylphenylhydrazine (which is known to precipitate haemolysis in sensitive subjects, and depletes glutathione) methylene blue (which oxidises NADPH) All incubations were repeated in the presence and absence of N -ethylmaleimide, which undergoes a non-enzymic reaction with the -SH group of reduced glutathione, and thus depletes the cell of glutathione

The Role of Precursors Sometimes, the best way to support a system is to provide the "building blocks." Compounds like N-acetyl cysteine (NAC) are precursors to glutathione

Although GSH is present in the extracellular fluid, it cannot be directly uptaken by cells [1, 2]