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Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 l volume containing 7.5 l eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 l 5 RT buffer (Promega), 30 U RNasin RNase inhibitor (Promega), and 0.75 g random hexadeoxynucleotides (Roche) in order to retrotranscribe the whole HCV genome preventing any bias during the reaction

These elevations may also contribute to enhanced downstream signalling of Gq through activation of phospholipase C (PLC), as has been reported in INS-1 cells [22]

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Yousefi, M