Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT
Ancillary action has also been reported
Clinicians should be aware that even experienced radiologists will commonly label a small focus of hepatic metastasis, defined by contrast-enhanced CT scanning, as a hemangioma
The mismatch isn't fatal to results, but it's suboptimal