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The Collaborative of Eating Disorder Organizations wrote an open letter to the AAP, which said in part: "The statements throughout these guidelines are problematic at best, and at worst, put American children and adolescents at serious risk for developing eating disorders, disordered eating, and other mental and physical health issues." Bolling says hes unaware if there is a relationship between GLP-1 agonist medications and eating disorders but acknowledges more research needs to be done on this topic

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After washing in ultrapure water, the sections were heated at 60 C, air dried, and incubated in xylene for 5 min