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doi: 10.17235/reed.2024.10305/2024 106 DesaiAKhataniarHHashashJGFarrayeFARegueiroMKochharGS

Oxidation kinetics for roGFP2 WT -PfGrx fusion constructs The in vivo oxidation kinetics from fusion constructs between roGFP2 WT and a variety of glutaredoxins and mutants thereof were shown to correlate very well with the rate constants for the oxidative half-reaction from GSSCys assays in vitro, suggesting that the glutathionylation of the glutaredoxin and not the transfer of oxidation to the fused roGFP2 moiety was rate-limiting under the chosen conditions 15,34,35

In details, the antibodies used for western blot experiments were Pan-Akt Polyclonal Antibody (E-AB-30471, Elabscience), Phospho-Pan-Akt (Ser473) Polyclonal Antibody (E-AB-20802, Elabscience) and anti--Tubulin (sc-32293, Santa Cruz Biotechnology)

In normal physiology, ammonium derived from deamination is transported as glutamine or alanine to hepatocyte mitochondria, where it undergoes enzymatic conversion to urea via the urea cycle (Details in Table 1 )