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Afterward, the samples were centrifuged for 10 min at 16000 g at 4 C, and the supernatant was transferred to an autosampler vial and dried with a gentle stream of N2

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The mass spectrometer was operated in the positive electrospray ionization (ESI) mode, and the MS/MS analysis was performed in the multiple reaction monitoring (MRM) mode, monitoring the transitions of m/z 162.0 103.0 for carnitine, m/z 147.1 88.1 for IS, respectively

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