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Tissue remodeling continues for weeks after pain resolves
While previous imaging techniques have mainly focused on magnetic resonance spectroscopy (MRS), which detects localized concentrations of metabolites such as choline, citrate, and various amino acids, these metabolic profiles reflect tumor metabolic intensity and may indicate responsiveness to ammonium-targeted therapies (278, 279)
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Cellular cAMP determination assay Freshly isolated CSMCs were cultured in DMEM supplemented with 10% FBS, 100 U/mL of penicillin, and 100 g/mL of streptomycin at 37 C in 5% CO 2
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