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(55) found that in a mouse model of corneal alkali injury, the expression of ROS was upregulated, oxidative stress increased, the expression of the gene Ptgs2, a key marker of ferroptosis, was elevated, the level of the lipid peroxidation product 4-hydroxynonenal (4-HNE) increased, and the level of the ferroptosis regulator GPX4 decreased, with mitochondrial damage

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GSTP1 Activity Assay HepG2 cells were plated on collagen-coated 6-well plates at 3 10 5 cells per well and transfected as described in the above section

Suarez, G