Equal amounts of protein were resolved on SDS-polyacrylamide gel, transferred to nitrocellulose membrane (Amersham Biosciences, Milan, Italy), and then treated with the following primary antibodies: anti-Atg5 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-Atg7 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-LC3A/B rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-SQSTM1/p62 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-Beclin-1 rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-Phospho-S6 Ribosomal Protein rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-S6 Ribosomal Protein mouse monoclonal (Cell Signaling Technology, MA, USA), anti-Phospho-AMPK rabbit monoclonal (Cell Signaling Technology, MA, USA), anti-AMPK mouse polyclonal (Cell Signaling Technology, MA, USA), anti-Cyclin D1 (Dako, Milan, Italy)
Prescribing regulations for research peptides vary by jurisdiction, but in most cases, doctors cannot legally prescribe research-grade compounds for human therapeutic use
Free Radic Biol Med 51: 194351
It might just mean the dose needs to be adjusted, or that youre still in the early stages
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