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7), coinciding with a tirzepatide dose increase that occurred before surgical implantation

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coli BL21 (DE3) as fusion proteins carrying a C-terminal intein-chitin binding domain (intein-CBD) and affinity purified in binding buffer (20 mM HEPES, Na-acetate pH 6.5, 75 mM NaCl) on a chitin matrix gravity flow column (New England Biolabs, Frankfurt/Main DE, cat

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