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+ -dependent deacetylase sirtuin-1 (SIRT1) meticulously regulates a variety of substrates, such as histone and non-histone proteins, thereby producing significant effects on metabolic homeostasis, apoptosis regulation, inflammatory responses, attenuation of oxidative stress, and even the aging process (Tanno et al., 2007

the substantial amount is at least 70 - 80 % in 10 - 12 hrs, preferably at least 90 % in 12 hrs and most preferably at least 95 % in 12 hrs

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