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In VRK2-WT cells, as expected, most of SLC7A11 was redistributed to the ER after 1 h of BFA treatment, while at 4 h after BFA removal, almost all the cells showed co-localization of SLC7A11 and the Golgi marker Golgin-97

Mao X, et al

Or, even better, write the discard after date, which is 28 days from the opening date

For example, herbivore-induced plant volatile compounds could serve as repellents of some insects and reduce their activities, which is termed allelochemical nonpreference [4]