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Thus, while we were not successful in obtaining more reactive analogs at this point, this approach allows us to explain the observed low reactivity at physiological pH and offers a valuable tool for guiding the future design of nucleotide-based inhibitors with optimized preorientation and reactivity profiles, providing a systematic framework for enhancing these compounds reactivities in a targeted manner

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Conversely, ectopic expression of the Ubx N51A did not impact the identity of thoracic muscles, illustrating its inability to induce homeotic transformation by itself (Figure 1F-G)

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