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Description

Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

Prolonged suppression can lead to erosion of pituitary function altogether, a condition described as disuse atrophy (analogous to an unused muscle)

Ewes were synchronized for estrus using the OvSynch protocol and a dose of LC (20 mg/kg body weight) was intravenously (IV) administered on the same day of PGF 2 injection to one group (n = 8