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BODIPY staining To assess differentiation potential, fully differentiated adipocytes were stained with 1 M BODIPY for 20 min, followed by washing with PBS and imaged using the PerkinElmer Operetta high-content imaging system

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Of note, the relative abundance of the Parasutterella genus, which was significantly enriched in both the vehicle- and 5A-1MQ-treated animals transitioned from WD to LD compared to both the WD and LD control groups, was observed to be significantly correlated (either positively or negatively) with 30 EWAT metabolites, both with and without outliers included

A reconstituted vial left at room temperature for 24 hours experiences degradation equivalent to approximately 8 days of refrigerated storage