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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

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Step-by-step guide to resuming your program When you are ready to move forward, you should follow a structured approach to ensure your body tolerates the medication

Consult your doctor before pausing any medication, and understand the impact it may have on your health