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Description

Co-expression cell pellets were resuspended in 3 mL assay buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol) and this was then divided into 1.5 mL for each of the different affinity pull-downs

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116 MalekiS.KusturicaJ.GuiE.Rakanovi-TodiM.eiD.Burnazovi-RistiL.et al (2017)

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