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Alpha-1antitrypsin: a polyfunctional protein

Cells were then treated with 100 mM reducing agent sodium 2-mercaptoethanesulfonate (Mesna) in TNE buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 8.6) or with TNE buffer alone for 5 minutes before being washed 1x in PBS and imaged in Hanks Balanced Salt Solution (HBSS) buffer on a Nikon Ti2E wide-field microscope with a light-emitting diode (LED) light source and a 20x/0.75 NA objective, with several phase contrast and epifluorescence images acquired per well using corresponding filters

The study also reported that participants who took the prickly pear supplement experienced an improvement in gastrointestinal symptoms, suggesting that positive changes in the gut microbiota correlated with a reduction in common digestive issues like bloating and pain

Research suggests it may modulate the expression of genes involved in tissue remodeling, support collagen and elastin synthesis, and activate antioxidant and anti-inflammatory pathways