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Cryo-EM Three microliters of the purified GLP-2GLP-2RG s complex at about 20 mg/mL was applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), blotted, and subsequently sample-coated grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific, Waltham, USA)
Remember to always use a new needle for each shot
The patients also demonstrated effects from the drug noted in previous studies of people without HIV, that included weight loss, reduced fasting blood glucose and reduced fasting triglycerides
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