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nsMRA, nonsteroidal mineralocorticoid receptor antagonist

A parallel plate treated identically was incubated for an additional 3 h in fresh assay medium (complete culture medium, without FBS, G418 Sulfate, and Blasticidin S HCl, supplemented with 1 Eagles MEM Non-Essential Amino Acids Solution

Additionally, information regarding the funding sources of the studies has been included in the Supplementary Table S1 (summary of study funding sources)

Juvenile and adult worms were carefully washed in phenol red free RPMI medium and subsequently incubated in phenol red free RPMI medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and at 37 C in a 5% CO 2 incubator overnight