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The elution followed a linear gradient at a flow rate of 0.3 mL/min under the following conditions: 520% B from 0 to 1 min, 2025% B from 1 to 5.5 min, 2530% B from 5.5 to 6 min, 30% B from 6 to 8 min, 3035% B from 8 to 9 min, 3565% B from 9 to 17 min, 65100% B from 17 to 18 min, 100% B from 18 to 19 min, and 5% B from 19.1 to 20 min

Mass spectrometry imaging (MSI): Matrix-assisted laser desorption/ionization (MALDI) MSI allows researchers to map glutathione distribution across tissue sections with spatial resolution on the order of tens of micrometers

5 Conclusion CHF combining RG showed better therapeutic effects than RG alone, and its renal protective effects were associated with reducing Scr, BUN, UA, and improving eGFR, as well as preventing renal fibrosis

Sprays, patches, sublinguals etc