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Plasma protein extraction and digestion For the discovery cohort (2011), two separate preparations were made with either 1 or 2 L of plasma added to new tubes containing 2423 L of SDC buffer consisting of sodium deoxycholate 1% (DOC), tris(2-carboxyethyl) phosphine 10 mM (TCEP), chloroacetamide 40 mM and Tris 100 mM pH 8.5

This bifurcation Akt/mTORC1 for protein synthesis, ERK1/2 for proliferation represents the canonical dual anabolic output of IGF-1R activation, and both arms are relevant to skeletal muscle research depending on the biological question (hypertrophy vs regeneration)

Subsequent work by Heffernan and coworkers (2001) confirmed the peptides effects in obese mouse models, with chronic administration associated with reduced body weight gain and decreased adiposity without concurrent changes in food intake