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Each cell suspension was directly loaded onto a microwell cartridge of the BD Rhapsody Express system (BD Biosciences, #400000847) and libraries were prepared using the BD Rhapsody WTA Reagent kit (BD Biosciences, #633802) following the manufacturers instructions

The addition of the glucagon receptor absent from all other approved or late-stage metabolic peptides activates energy expenditure pathways that the GLP-1 and GIP receptors alone do not reach

GLP-1 medications are prescription drugs that help control appetite and blood sugar levels

These areas were selected because they have enough subcutaneous fat for safe, effective medication absorption while being relatively accessible and comfortable