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The in vivo model utilized 10-week-old male C57BL/6 mice that were housed in environmentally controlled conditions with a 12-hour dark-light cycle, receiving standard laboratory chow and water ad libitum

Additionally, dysregulation of key neurotransmitters, such as elevated noradrenaline and reduced serotonin, particularly within the amygdala, has been implicated in heightened anxiety and impaired stress resilience (112, 113)

Integrative translational approaches, which combine a range of investigative methodologies including neuroimaging, metabolic profiling, and inflammatory markers, could lead to new avenues of study and offer new insights into the detrimental effects of UPFs, as well as the efficacy of potential therapeutic interventions