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The progression over 24 months of follow-up of ALSFRS-R total score and FVC% were evaluated using repeated measures linear mixed models with random intercept and slope, with, separately, ALSFRS and FVC% as dependent variable and treatment (not treated or treated with ALCAR), time (month 024) and treatment time interaction as independent variables

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The sustained effects, lasting months after treatment cessation, suggest that cerebrolysin triggers structural brain changes rather than providing only temporary pharmacological effects

In contrast, PKLR was upregulated while no difference in the levels of PFK-1 was observed in ibuprofen treated female livers