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The microbiota regulates the expression of tight junction proteins (such as ZO-1 and occludin) in intestinal epithelial cells by releasing specific metabolites and signaling molecules, thereby safeguarding the structural and functional integrity of the intestinal barrier ( Studies demonstrate that when TLR5 / germ-free mice are infected with adherent-invasive Escherichia coli (AIEC) and subsequently exposed to a specific SPF microbial environment, they develop colitis symptoms ( The gut microbiota of IBD patients exhibits a trend towards reduced diversity, manifested explicitly by a decrease in the abundance of commensal Clostridia and a significant enrichment of pathogenic bacteria

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