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Quantitative RT-PCR RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA) and was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen, Chatsworth, CA, USA)

Some of the women were also treated with intravenous glutathione

Gut microbiota acts as a common mediator for key nutrients [e.g., fiber, Omega-3, zinc], which exert antidepressant effects via shared pathways (enhancing beneficial bacteria, promoting SCFAs, reducing inflammation, regulating the gut-brain axis) and nutrient-specific regulation [e.g., Omega-3 enriching Roseburia, fiber boosting SCFA-producing bacteria]

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